cas9 expressing vector Search Results


all-in-one  (Genecopoeia)
99
Genecopoeia all-in-one
All In One, supplied by Genecopoeia, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/all-in-one/product/Genecopoeia
Average 99 stars, based on 1 article reviews
all-in-one - by Bioz Stars, 2026-03
99/100 stars
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sgrna/cas9 expression vectors  (Guangzhou Fulengen Co)
90
Guangzhou Fulengen Co sgrna/cas9 expression vectors
The linear map of <t>CRISPR/Cas9</t> vector (A) and the locations and sequences of the three sgRNAs targeting the fourth and fifth exons of ABCB1 gene (B)
Sgrna/Cas9 Expression Vectors, supplied by Guangzhou Fulengen Co, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/sgrna/cas9 expression vectors/product/Guangzhou Fulengen Co
Average 90 stars, based on 1 article reviews
sgrna/cas9 expression vectors - by Bioz Stars, 2026-03
90/100 stars
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crispr/cas9 expression vector  (TransGen biotech co)
90
TransGen biotech co crispr/cas9 expression vector
The vector pBSE401 used for <t>CRISPR/Cas9-mediated</t> genome editing. AtU6, Arabidopsis U6 promotor; gRNA, guide RNA; 35S promotor, CaMV 35S promotor; Cas9, codon-optimized Cas9; NLS, nuclear location signal; bar , selective marker gene; KanR, Kanamycin resistance gene; pVS1-RepA, pVS1 replication origin; pVS1-StaA, pVS1 stability function.
Crispr/Cas9 Expression Vector, supplied by TransGen biotech co, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/crispr/cas9 expression vector/product/TransGen biotech co
Average 90 stars, based on 1 article reviews
crispr/cas9 expression vector - by Bioz Stars, 2026-03
90/100 stars
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Image Search Results


The linear map of CRISPR/Cas9 vector (A) and the locations and sequences of the three sgRNAs targeting the fourth and fifth exons of ABCB1 gene (B)

Journal: Iranian Journal of Basic Medical Sciences

Article Title: CRISPR/Cas9, a new approach to successful knockdown of ABCB1/P-glycoprotein and reversal of chemosensitivity in human epithelial ovarian cancer cell line

doi: 10.22038/IJBMS.2017.25145.6230

Figure Lengend Snippet: The linear map of CRISPR/Cas9 vector (A) and the locations and sequences of the three sgRNAs targeting the fourth and fifth exons of ABCB1 gene (B)

Article Snippet: Three custom-designed sgRNA/Cas9 expression vectors, targeting the fourth and fifth exons of ABCB1 gene (Accession No NM_000927.3), were designed using CRISPR DESIGN ( http://crispr.mit.edu/ ) tool and were then synthesized by Guangzhou FulenGen Company (P R China).

Techniques: CRISPR, Plasmid Preparation

Real-time Q-PCR analysis of expression of ABCB1. The mRNA levels of ABCB1 were analyzed by real-time PCR, 72 hr after antibiotic selection. Expression levels of ABCB1 were normalized to an average of two reference genes, GAPDH and β-Actin. A2780/ADR cells transfected with CRISPR/Cas9 showed a remarkable decrease in ABCB1 expression level compared to the control and scramble groups; bars: SE (***, P< 0.001 )

Journal: Iranian Journal of Basic Medical Sciences

Article Title: CRISPR/Cas9, a new approach to successful knockdown of ABCB1/P-glycoprotein and reversal of chemosensitivity in human epithelial ovarian cancer cell line

doi: 10.22038/IJBMS.2017.25145.6230

Figure Lengend Snippet: Real-time Q-PCR analysis of expression of ABCB1. The mRNA levels of ABCB1 were analyzed by real-time PCR, 72 hr after antibiotic selection. Expression levels of ABCB1 were normalized to an average of two reference genes, GAPDH and β-Actin. A2780/ADR cells transfected with CRISPR/Cas9 showed a remarkable decrease in ABCB1 expression level compared to the control and scramble groups; bars: SE (***, P< 0.001 )

Article Snippet: Three custom-designed sgRNA/Cas9 expression vectors, targeting the fourth and fifth exons of ABCB1 gene (Accession No NM_000927.3), were designed using CRISPR DESIGN ( http://crispr.mit.edu/ ) tool and were then synthesized by Guangzhou FulenGen Company (P R China).

Techniques: Expressing, Real-time Polymerase Chain Reaction, Selection, Transfection, CRISPR

Detection of indels generated by CRISPR/Cas9 using surveyor assay. Red arrowheads indicate predicted Cas9 cutting sites of ABCB1 PCR products., sgRNA-1: PCR product size: 510 bp, predicted cleavage size: 220 & 290 bp, sgRNA-2: PCR product size: 510 bp, predicted cleavage size: 170 & 340 bp, sgRNA-3: PCR product size: 952 bp: Predicted cleavage size: 335 & 617 bp, Control-1: 510 bp, Control-2: 952 bp

Journal: Iranian Journal of Basic Medical Sciences

Article Title: CRISPR/Cas9, a new approach to successful knockdown of ABCB1/P-glycoprotein and reversal of chemosensitivity in human epithelial ovarian cancer cell line

doi: 10.22038/IJBMS.2017.25145.6230

Figure Lengend Snippet: Detection of indels generated by CRISPR/Cas9 using surveyor assay. Red arrowheads indicate predicted Cas9 cutting sites of ABCB1 PCR products., sgRNA-1: PCR product size: 510 bp, predicted cleavage size: 220 & 290 bp, sgRNA-2: PCR product size: 510 bp, predicted cleavage size: 170 & 340 bp, sgRNA-3: PCR product size: 952 bp: Predicted cleavage size: 335 & 617 bp, Control-1: 510 bp, Control-2: 952 bp

Article Snippet: Three custom-designed sgRNA/Cas9 expression vectors, targeting the fourth and fifth exons of ABCB1 gene (Accession No NM_000927.3), were designed using CRISPR DESIGN ( http://crispr.mit.edu/ ) tool and were then synthesized by Guangzhou FulenGen Company (P R China).

Techniques: Generated, CRISPR

Doxorubicin chemosensitivity. MTT assay was performed to evaluate A2780/ADR cells viability, transfected and untransfected cells (control), 48 hr post-treatment with increasing concentrations of doxorubicin. The optical density of each well was measured with a microplate spectrophotometer at 490 nm. The viability of cells transfected with CRISPR/Cas9 was dramatically decreased compared to the control and scrambled transfected cells. In all groups, the proliferation rate of the cells was decreased with increasing concentrations of doxorubicin. The greatest decrease in proliferation rate was observed in A2780/ADR cells transfected with CRISPR/Cas9 compared to other two groups. Each condition was repeated three times (***, P <0.001)

Journal: Iranian Journal of Basic Medical Sciences

Article Title: CRISPR/Cas9, a new approach to successful knockdown of ABCB1/P-glycoprotein and reversal of chemosensitivity in human epithelial ovarian cancer cell line

doi: 10.22038/IJBMS.2017.25145.6230

Figure Lengend Snippet: Doxorubicin chemosensitivity. MTT assay was performed to evaluate A2780/ADR cells viability, transfected and untransfected cells (control), 48 hr post-treatment with increasing concentrations of doxorubicin. The optical density of each well was measured with a microplate spectrophotometer at 490 nm. The viability of cells transfected with CRISPR/Cas9 was dramatically decreased compared to the control and scrambled transfected cells. In all groups, the proliferation rate of the cells was decreased with increasing concentrations of doxorubicin. The greatest decrease in proliferation rate was observed in A2780/ADR cells transfected with CRISPR/Cas9 compared to other two groups. Each condition was repeated three times (***, P <0.001)

Article Snippet: Three custom-designed sgRNA/Cas9 expression vectors, targeting the fourth and fifth exons of ABCB1 gene (Accession No NM_000927.3), were designed using CRISPR DESIGN ( http://crispr.mit.edu/ ) tool and were then synthesized by Guangzhou FulenGen Company (P R China).

Techniques: MTT Assay, Transfection, Spectrophotometry, CRISPR

The vector pBSE401 used for CRISPR/Cas9-mediated genome editing. AtU6, Arabidopsis U6 promotor; gRNA, guide RNA; 35S promotor, CaMV 35S promotor; Cas9, codon-optimized Cas9; NLS, nuclear location signal; bar , selective marker gene; KanR, Kanamycin resistance gene; pVS1-RepA, pVS1 replication origin; pVS1-StaA, pVS1 stability function.

Journal: Frontiers in Plant Science

Article Title: Creation of Early Flowering Germplasm of Soybean by CRISPR/Cas9 Technology

doi: 10.3389/fpls.2019.01446

Figure Lengend Snippet: The vector pBSE401 used for CRISPR/Cas9-mediated genome editing. AtU6, Arabidopsis U6 promotor; gRNA, guide RNA; 35S promotor, CaMV 35S promotor; Cas9, codon-optimized Cas9; NLS, nuclear location signal; bar , selective marker gene; KanR, Kanamycin resistance gene; pVS1-RepA, pVS1 replication origin; pVS1-StaA, pVS1 stability function.

Article Snippet: And then the CRISPR/Cas9 expression vector was transformed into E. coli Trans1 T1 (TransGen Biotech) used for soybean genetic transformation.

Techniques: Plasmid Preparation, CRISPR, Marker

Identifying transplants in T 0 generation. (A) Detection of the selectable marker gene bar by PAT/Bar test strip. The red arrowhead indicates that bar gene is positive. (B) Gel image of PCR products for T-DNA regions. Cas9, part of the Cas9 coding sequence. sgRNA, region from the U6 promoter to the downstream vector sequence spanning the sgRNA. GmActin was used as a normalization control. V: plasmid of the vector in transformation. WT, wild type soybean plants. Labels 1-16, individual mutant lines.

Journal: Frontiers in Plant Science

Article Title: Creation of Early Flowering Germplasm of Soybean by CRISPR/Cas9 Technology

doi: 10.3389/fpls.2019.01446

Figure Lengend Snippet: Identifying transplants in T 0 generation. (A) Detection of the selectable marker gene bar by PAT/Bar test strip. The red arrowhead indicates that bar gene is positive. (B) Gel image of PCR products for T-DNA regions. Cas9, part of the Cas9 coding sequence. sgRNA, region from the U6 promoter to the downstream vector sequence spanning the sgRNA. GmActin was used as a normalization control. V: plasmid of the vector in transformation. WT, wild type soybean plants. Labels 1-16, individual mutant lines.

Article Snippet: And then the CRISPR/Cas9 expression vector was transformed into E. coli Trans1 T1 (TransGen Biotech) used for soybean genetic transformation.

Techniques: Marker, Stripping Membranes, Sequencing, Plasmid Preparation, Transformation Assay, Mutagenesis

CRISPR/Cas9-mediated targeted mutants of E1 in the T 1 generation.

Journal: Frontiers in Plant Science

Article Title: Creation of Early Flowering Germplasm of Soybean by CRISPR/Cas9 Technology

doi: 10.3389/fpls.2019.01446

Figure Lengend Snippet: CRISPR/Cas9-mediated targeted mutants of E1 in the T 1 generation.

Article Snippet: And then the CRISPR/Cas9 expression vector was transformed into E. coli Trans1 T1 (TransGen Biotech) used for soybean genetic transformation.

Techniques: CRISPR, Mutagenesis

CRISPR/Cas9-induced E1 mutants flowering time under both LD and SD conditions. (A) Phenotypes of wild type (WT, Jack) and homozygous T 2 mutant under LD condition, respectively. Top panel, WT did not have floral buds when T 2 mutant was flowering. Bottom panel, T 2 mutant produced the pods when WT was flowering. Red box, magnified view. (B) Flowering time of WT and homozygous T 2 mutants under LD condition. (C) Phenotypes of wild type (WT, Jack) and homozygous T 2 mutant under SD condition, respectively. (D) Flowering time of WT and homozygous T 2 mutants under SD condition. n, exact numbers of individual plants identified. **, homozygous T 2 mutants exhibit significant early flowering time (P < 0.01). The flowering time is shown as the mean values ± standard deviation.

Journal: Frontiers in Plant Science

Article Title: Creation of Early Flowering Germplasm of Soybean by CRISPR/Cas9 Technology

doi: 10.3389/fpls.2019.01446

Figure Lengend Snippet: CRISPR/Cas9-induced E1 mutants flowering time under both LD and SD conditions. (A) Phenotypes of wild type (WT, Jack) and homozygous T 2 mutant under LD condition, respectively. Top panel, WT did not have floral buds when T 2 mutant was flowering. Bottom panel, T 2 mutant produced the pods when WT was flowering. Red box, magnified view. (B) Flowering time of WT and homozygous T 2 mutants under LD condition. (C) Phenotypes of wild type (WT, Jack) and homozygous T 2 mutant under SD condition, respectively. (D) Flowering time of WT and homozygous T 2 mutants under SD condition. n, exact numbers of individual plants identified. **, homozygous T 2 mutants exhibit significant early flowering time (P < 0.01). The flowering time is shown as the mean values ± standard deviation.

Article Snippet: And then the CRISPR/Cas9 expression vector was transformed into E. coli Trans1 T1 (TransGen Biotech) used for soybean genetic transformation.

Techniques: CRISPR, Mutagenesis, Produced, Standard Deviation

Identifying of trans-clean mutants. (A) Detection of the selectable marker gene bar by PAT/Bar test strip. The red arrowhead indicates that bar gene is positive. (B) Gel image of PCR products for T-DNA elements. Cas9, part of the Cas9 coding sequence. sgRNA, region from the U6 promoter to the downstream vector sequence spanning the sgRNA. GmActin was used as a normalization control. V: plasmid of the vector in transformation. WT, wild type. Labels 1-20, individual mutant lines.

Journal: Frontiers in Plant Science

Article Title: Creation of Early Flowering Germplasm of Soybean by CRISPR/Cas9 Technology

doi: 10.3389/fpls.2019.01446

Figure Lengend Snippet: Identifying of trans-clean mutants. (A) Detection of the selectable marker gene bar by PAT/Bar test strip. The red arrowhead indicates that bar gene is positive. (B) Gel image of PCR products for T-DNA elements. Cas9, part of the Cas9 coding sequence. sgRNA, region from the U6 promoter to the downstream vector sequence spanning the sgRNA. GmActin was used as a normalization control. V: plasmid of the vector in transformation. WT, wild type. Labels 1-20, individual mutant lines.

Article Snippet: And then the CRISPR/Cas9 expression vector was transformed into E. coli Trans1 T1 (TransGen Biotech) used for soybean genetic transformation.

Techniques: Marker, Stripping Membranes, Sequencing, Plasmid Preparation, Transformation Assay, Mutagenesis